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洪士杰 Hung, Shih-Chieh

教授 / 中國醫藥大學生物醫學研究所

主治醫師 / 台北榮民總醫院骨科部運動醫學科

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[ CV ]
  • Email: hung3340@gmail.com
  • Tel: 04-22052121#7728
  • EDUCATION AND POSITIONS HELD:

    • M.D. National Yang-Ming University (1983-1990)
    • Ph.D. The University of Tokyo (1993-1997)
    • Residency, Surgical Department, Fan-Lin Veterans Hospital, Hwa-Ling, Taiwan (1990/7~1992/7)
    • Residency Training: Department of Orthopaedics and Traumatology, Veterans General Hospital-Taipei (1992/7~2000/6)
    • Research Fellowship: Department of Orthopaedics, School of Medicine, The University of Tokyo (1993/7~1997/6)
    • Postdoctoral Fellowship: Department of Biochemistry, The University of Tokyo (1996/8~1997/6)
    • Visiting Investigator: Biomedical Institute, Sinica Academy (2003/1~2003/12)
    • Senior Research Scientist: Center for Gene Therapy, Tulane University Health Science Center: Prof. Prockop-DJ (2004/8-2005/8)
    • Attending Physician, Department of Orthopedics, Taipei Veterans General Hospital, 2000-present
    • Attending Physician, Department of Medical and Education Research, Taipei Veterans General Hospital, 2002-present
    • Professor, Institute of Clinical Medicine, Natl. Yang-Ming Univ., Taipei,Taiwan, 2007-present

    HONORS:

    • National Scholarship for overseas education 1993-1997
    • Award, Japanese Medical Association 1996
    • The best research award, Taiwan Orthopedic Association 2001
    • The best scientific paper award, Taipei Veterans General Hospital 2002
    • Outstanding award, National Yang-Ming University 2007-2010
    • The 2nd Award for Medical Technology Innovation, Taipei Veterans General hospital, 2010
    • Award, 1st place, Cumulative SCI IF, Taiwan Orthopaedic Research Society, 2011
    • The 1st place, best scientific paper award, Taipei Veterans General Hospital 2012
    • Award, 1st place, Cumulative SCI IF, Taiwan Orthopaedic Research Society, 2012

    RESEARCH INTERESTS:

    We have focused on the investigation about mesenchymal stem cells (MSCs) and the molecular pathways involved in tumor initiation and chemoresistance of cancer stem cells (CSCs). We have published up to 80 papers on stem cell and related studies. The most current breakthrough is the expansion of human bone marrow MSCs under hypoxic conditions. Since bone marrow is hypoxic, the original niches or microenvironments of MSCs are under hypoxic condition with the oxygen tension around 1 to 7%. We aim to study the effects of hypoxic culture on MSCs. Hypoxic culture increases the expression of Oct4 and Nanog, which upregulate Dnmt1, thereby maintaining stem cell properteis and undifferentiated status (Molecular Cell, 47: 169–182, 2012). HIF-1 upregulated under hypoxic conditions activates Twist to inhibit E2A-p21 pathway, thereby preventing replicative senescence (Blood 117:459-69. 2011). Furthermore, hypoxic culture increases the in vitor migration and in vivo engraftment of MSCs after transplantation, and increases the angiogenic factors such as VEGF secreted by MSCs. We have also published 10 more papres in Stem cells, Aging Cell, PLoS ONE and Cell Transplantation. We have also filed several patent applications. These results willl help to develop new stratedge in cell therapy. We have also investiagted the relationships between MSCs and cancer stem cells from clorecal cancer and lung cancer. These papers have been publsihed in Gastroenterology, Oncogene and Cancer.

    研究室長期致力於人類骨髓間葉幹細胞的研究,近來也著重於癌症幹細胞的分子探討,過去有將近 80 篇幹細胞相關論文發表。最近的突破為利用缺氧的環境來培養人類骨髓間葉幹細胞。利用低氧環境增殖的骨髓間葉幹細胞,可以表現胚胎幹細胞基因,如Oct4及Nanog,這些基因可以透過促進Dnmt1之表現來維持幹細胞的分裂及未分化狀態 (Molecular Cell, 47: 169–182, 2012); 低氧也可以透過HIF-1轉錄因子,來促進Twist表現,進而調降E2A-p21以抑制細胞因細胞分裂所造成老化現象(Blood 117:459-69. 2011);同時間葉幹細胞於低氧狀況下也會表現較多的趨附因子受器,因而增加移植後移動及著床的能力。且分泌更多和血管新生或組織再生的因子,因而可以促進損傷組織的修復。除此之外,低氧氣濃度下培養增殖骨髓間葉幹細胞相關研究,實驗結果發現低氧培養可以增進間葉幹細胞的很多效能。相關成果也已經整理成10篇論文發於「Stem Cells」, 「Aging Cell」,「PLoS ONE」「Cell Transplantation」等雜誌。相關研究成果也申請本國及美國專利中。這些研究成果說明低氧培養可以提升以間葉幹細胞做為新式治療之成功發展。我們也探討間葉幹細胞和大腸癌及肺癌幹細胞的相關性,這些論文也先後發表於「Gastroenterology」,「Oncogene」「Cancer」等雜誌。

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