李光申 Lee, Oscar Kuang-Sheng
講座教授 / 陽明臨床醫學研究所![]() [ CV ]
Email: kslee@vghtpe.gov.tw
Tel: 02-28757391
EDUCATION AND POSITIONS HELD:
HONORS:
RESEARCH INTERESTS:The major research theme of Dr. Lee’s lab is plasticity and application of MSCs. Being an orthopaedic surgeon as well as a stem cell scientist, Dr. Lee is particularly interested in developing new application of MSCs to treat orthopaedic problems. The research interests also include osteoporosis, bone cell biology, and biophysical effects on bone cells and stem cells, and bone tumor biology. The lab has pioneered to successfully isolate MSCs not only from human bone marrow but also from human umbilical cord blood, human parathyroid gland, fat tissues and cruciate ligaments. In addition, Dr. Lee’s group is among the first to demonstrate the differentiation potential of MSCs into hepatocytes as well as the feasibility of using MSCs to treat fulminant hepatic failure. They also worked on an animal model of in utero transplantation of human MSCs into mice to investigate their differentiation potentials in vivo.
Human bone marrow MSCs Umbilical cord blood MSCs ![]() ![]() Dr. Lee has studied extensively the mechanisms governing the proliferation and differentiation process of MSCs. Regarding the proliferation ability, they demonstrated the advantages of low oxygen and low glucose culture condition, and the disadvantages of cell contact of MSCs during maintenance. Regarding the differentiation potential, they further investigated the effects of using electrospun type I collagen nanofibers as a scaffold for tissue engineering, the effects of concomitant overexpression of Cbfb and Cbfa-1, the effects of matrix stiffness, the changes of mitochondrial biogenesis, and the changes of phosphoprotein expression during osteogenic differentiation. In addition, they developed a surface plasmon resonance biosensor that potentiates the real-time detection of MSCs during osteogenic differentiation. Furthermore, Dr. Lee’s lab has also made great efforts studying the role of hMSCs in regulating hematopoietic stem cell fate and their therapeutic potential to treat lupus nephritis, spinocerebellar ataxia and diabetes in different animal models. ![]() 瀏覽數
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